PUBLICATIONS

This is a full list of Alvaro’s publications.

2018

Integrative analysis of fitness and metabolic effects of plasmids in Pseudomonas aeruginosa PAO1.
San Millan A, Toll-Riera M, Qi Q, Betts A, Hopkinson RJ, McCullagh J, MacLean RC. ISME J. 2018 Aug 10. doi: 10.1038/s41396-018-0224-8.
Link: https://www.nature.com/articles/s41396-018-0224-8


Evolution of Plasmid-Mediated Antibiotic Resistance in the Clinical Context.
San Millan A. Trends Microbiol. 2018 Jul 23. doi: 10.1016/j.tim.2018.06.007.
Link: https://www.cell.com/trends/microbiology/fulltext/S0966-842X(18)30142-2


Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance.
Escudero JA, MacLean RC, San Millan A. J Vis Exp. 2018 May 2;(135). doi: 10.3791/57386.
Link: https://www.jove.com/video/57386/testing-role-multicopy-plasmids-evolution-antibiotic


Multicopy plasmids allow bacteria to escape from fitness trade-offs during evolutionary innovation.
Rodriguez-Beltran J, Hernandez-Beltran JCR, DelaFuente J, Escudero JA, Fuentes-Hernandez A, MacLean RC, Peña-Miller R, San Millan A. Nature Ecology and Evolution. 2018 Apr 9. doi: 10.1038/s41559-018-0529-z.
Link: https://t.co/LgTQrHb0V6


Cooperation, competition and antibiotic resistance in bacterial colonies.
Frost I, Smith WPJ, Mitri S, San Millan A, Davit Y, Osborne JM, Pitt-Francis JM, MacLean RC, Foster KR. ISME Journal. 2018 Mar 21. doi: 10.1038/s41396-018-0090-4.
Link: https://www.nature.com/articles/s41396-018-0090-4


PCR-Based Analysis of ColE1 Plasmids in Clinical Isolates and Metagenomic Samples Reveals Their Importance as Gene Capture Platforms.
Ares-Arroyo M, Bernabe-Balas C, Santos-Lopez A, Baquero MR, Prasad KN, Cid D, Martin-Espada C, San Millan A, Gonzalez-Zorn B. Frontiers in Microbiology. 2018 Mar 16;9:469. doi: 10.3389/fmicb.2018.00469.
Link: https://www.frontiersin.org/articles/10.3389/fmicb.2018.00469/full

 

2017

Fitness Costs of Plasmids: a Limit to Plasmid Transmission.
San Millan A, MacLean RC. Microbiol Spectr. 2017 Sep;5(5). doi: 10.1128/microbiolspec.MTBP-0016-2017.
Link: http://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MTBP-0016-2017


A Naturally Occurring Single Nucleotide Polymorphism in a Multicopy Plasmid Produces a Reversible Increase in Antibiotic Resistance.
Santos-Lopez A, Bernabe-Balas C, Ares-Arroyo M, Ortega-Huedo R, Hoefer A, San Millan A, Gonzalez-Zorn B. Antimicrob Agents Chemother. 2017 Jan 24;61(2). pii: e01735-16.
Link: https://www.ncbi.nlm.nih.gov/pubmed/27895020

 

2016

Multicopy plasmids potentiate the evolution of antibiotic resistance in bacteria.
San Millan A, Escudero JA, Gifford DR, Mazel D, MacLean RC. Nature Ecology and Evolution. 2016 Nov 7;1(1):10. doi: 10.1038/s41559-016-0010.
Link: https://www.ncbi.nlm.nih.gov/pubmed/28812563
– Selected by Faculty of 1000.
– Highlighted in Nature Microbiology


The Genomic Basis of Evolutionary Innovation in Pseudomonas aeruginosa.
Toll-Riera M, San Millan A, Wagner A, MacLean RC. PLoS Genet. 2016 May 5;12(5):e1006005.
Link: http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1006005

 

2015

Evaluating the effect of horizontal transmission on the stability of plasmids under different selection regimes.
Peña-Miller R, Rodríguez-González R, MacLean RC, San Millan A. Mobile Genetic Elements. 2015 May 21;5(3):1-5.
Link: http://www.tandfonline.com/doi/full/10.1080/2159256X.2015.1045115


Microbial Evolution: Towards Resolving the Plasmid Paradox (Dispatch).
MacLean RC, San Millan A. Current Biology. 2015 Aug 31;25(17):R764-7.
Link: http://www.cell.com/current-biology/fulltext/S0960-9822(15)00809-X


Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa.
San Millan A, Toll-Riera M, Qi Q, MacLean RC. Nature Communications. 2015 Apr 21;6:6845.
Link: http://www.nature.com/ncomms/2015/150421/ncomms7845/full/ncomms7845.html


Small-plasmid-mediated antibiotic resistance is enhanced by increases in plasmid copy number and bacterial fitness.
San Millan A, Santos-Lopez A, Ortega-Huedo R, Bernabe-Balas C, Kennedy SP, Gonzalez-Zorn B.  Antimicrobial Agents and Chemotherapy. 2015;59(6):3335-41.
Link: http://aac.asm.org/content/59/6/3335.long

 

2014

Positive selection and compensatory adaptation interact to stabilize non-transmissible plasmids.
San Millan A, Peña-Miller R, Toll-Riera M, Halbert ZV, McLean AR, Cooper BS, MacLean RC. Nature Communications. 2014 Oct 10;5:5208.
Link: http://www.nature.com/ncomms/2014/141010/ncomms6208/full/ncomms6208.html
-Highlighted in Nature Reviews in Microbiology.


Culturable aerobic and facultative bacteria from the gut of the polyphagic dung beetle Thorectes lusitanicus.
Hernández N, Escudero JA, San Millán Á, González-Zorn B, Lobo JM, Verdú JR, Suárez M. Insect Science. 2015 Apr;22(2):178-90.
Link: http://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12094/abstract;jsessionid=5BFAF95404297F06B44DAE74F346F18A.f03t03


Positive epistasis between co-infecting plasmids promotes plasmid survival in bacterial populations.
San Millan A, Heilbron K, MacLean RC. ISME Journal. 2014 Mar;8(3):601-12.
Link: http://www.nature.com/ismej/journal/v8/n3/full/ismej2013182a.html

 

2013

SatR is a repressor of fluoroquinolone efflux pump SatAB.
Escudero JA, San Millan A, Montero N, Gutierrez B, Ovejero CM, Carrilero L, González-Zorn B. Antimicrobial Agents and Chemotherapy. 2013 Jul;57(7):3430-3.
Link: http://aac.asm.org/content/57/7/3430.long

 

2012

Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae.
Søndergaard A, San Millan A, Santos-Lopez A, Nielsen SM, Gonzalez-Zorn B, Nørskov-Lauritsen N. Antimicrobial Agents and Chemotherapy. 2012 Sep;56(9):4958-60.
Link: http://aac.asm.org/content/56/9/4958.long


Fitness cost and interference of Arm/Rmt aminoglycoside resistance with the RsmF housekeeping methyltransferases.
Gutierrez B, Escudero JA, San Millan A, Hidalgo L, Carrilero L, Ovejero CM, Santos-Lopez A, Thomas-Lopez D, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2012 May;56(5):2335-41.
Link: http://aac.asm.org/content/56/5/2335.long

 

2011

Fluoroquinolone efflux in Streptococcus suis is mediated by SatAB and not by SmrA.
Escudero JA, San Millan A, Gutierrez B, Hidalgo L, La Ragione RM, AbuOun M, Galimand M, Ferrándiz MJ, Domínguez L, de la Campa AG, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2011 Dec;55(12):5850-60.
Link: http://aac.asm.org/content/55/12/5850.long


ArmA methyltransferase in a monophasic Salmonella enterica isolate from food.
Granier SA, Hidalgo L, San Millan A, Escudero JA, Gutierrez B, Brisabois A, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2011 Nov;55(11):5262-6.
Link: http://aac.asm.org/content/55/11/5262.long


Plasmid-borne 16S rRNA methylase ArmA in aminoglycoside-resistant Klebsiella pneumoniae in Poland.
Zacharczuk K, Piekarska K, Szych J, Jagielski M, Hidalgo L, San Millán A, Gutiérrez B, Rastawicki W, González-Zorn B, Gierczynski R. Journal of Medical Microbiology. 2011 Sep;60(Pt 9):1306-11
Link: http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.024026-0#tab2


Contribution of ROB-1 and PBP3 mutations to the resistance phenotype of a β-lactamase-positive amoxicillin/clavulanic acid-resistant Haemophilus influenzae carrying plasmid pB1000 in Italy.
San Millan A, Giufré M, Escudero JA, Hidalgo L, Gutierrez B, Cerquetti M, Gonzalez-Zorn B. Journal of Antimicrobial Chemotherapy. 2011 Jan;66(1):96-9.
Link: https://jac.oxfordjournals.org/content/66/1/96.long

 

2010

Haemophilus influenzae clinical isolates with plasmid pB1000 bearing blaROB-1: fitness cost and interspecies dissemination.
San Millan A, Garcia-Cobos S, Escudero JA, Hidalgo L, Gutierrez B, Carrilero L, Campos J, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2010 Apr;54(4):1506-11.
Link: http://aac.asm.org/content/54/4/1506.long

 

2009

Novel genetic environment of qnrB2 associated with TEM-1 and SHV-12 on pB1004, an IncHI2 plasmid, in Salmonella Bredeney BB1047 from Spain.
Gutierrez B, Herrera-Leon S, Escudero JA, Hidalgo L, Gonzalez-Sanz R, Arroyo M, San Millan A, Echeita MA, Gonzalez-Zorn B. Journal of Antimicrobial Chemotherapy. 2009 Dec;64(6):1334-6.
Link: https://jac.oxfordjournals.org/content/64/6/1334.long


Multiresistance in Pasteurella multocida is mediated by coexistence of small plasmids.
San Millan A, Escudero JA, Gutierrez B, Hidalgo L, Garcia N, Llagostera M, Dominguez L, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2009 Aug;53(8):3399-404.
Link: http://www.ncbi.nlm.nih.gov/pubmed/19528282


VanB-type Enterococcus faecium clinical isolate successively inducibly resistant to, dependent on, and constitutively resistant to vancomycin.
San Millan A, Depardieu F, Godreuil S, Courvalin P. Antimicrobial Agents and Chemotherapy. 2009 May;53(5):1974-82
Link: http://aac.asm.org/content/53/5/1974.long

 

2007

Beta-lactam resistance in Haemophilus parasuis is mediated by plasmid pB1000 bearing blaROB-1.
San Millan A, Escudero JA, Catalan A, Nieto S, Farelo F, Gibert M, Moreno MA, Dominguez L, Gonzalez-Zorn B. Antimicrobial Agents and Chemotherapy. 2007 Jun;51(6):2260-4.
Link: http://aac.asm.org/content/51/6/2260.long


First characterization of fluoroquinolone resistance in Streptococcus suis.
Escudero JA, San Millan A, Catalan A, de la Campa AG, Rivero E, Lopez G, Dominguez L, Moreno MA, Gonzalez-Zorn B. Antimicrob Agents Chemother. 2007 Feb;51(2):777-82.
Link: http://aac.asm.org/content/51/2/777.long